A series of isonicotinyl hydrazones and their 4- thiazolidinones have been synthesized by condensation of isonicotinic acid hydrazide with various aromatic aldehydes to yield Schiff’s bases, followed by the cyclocondensation of Schiff’s bases with 2-mercaptoacetic acid to yield their 4-thiazolidinones with promising anti-mycobacterial and cytotoxicity activity. All these compounds were evaluated for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv using the microplate Alamar Blue assay. Among the compounds synthesized, N-(4-oxo-2-strylthiazolidin-3-yl)isonicotinamide (5k) with a stryl ring at the C-2 position of the thiazolidinone ring and N-[2-(4-fluorophenyl)-4-oxo-1,3-thiazolidin-3-yl] pyridine-4-carboxamide (5i) were found to be the most potent compounds with minimum inhibitory concentrations of 4.92 µM and 9.84 µM respectively against Mycobacterium tuberculosis H37 Rv. In the cytotoxicity testing, compound 7f yielded good antiproliferative activity when tested against MDA-MB cell line by MTT assay with GI50 at 131.58µg/ml. The cell viability of the tested compounds determined on Mouse Embryo Cell Line NIH /3T3 indicated that there was no lytic activity at the tested concentration interval of 100-300µg /ml, thereby demonstrating that the compounds were not cytotoxic to the host cell at these concentrations.
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